A new solid medium for isolating and enumerating Thiobacillus ferrooxidans.
نویسندگان
چکیده
منابع مشابه
Thiobacillus ferrooxidans, a facultative hydrogen oxidizer.
The type strain (ATCC 23270) and two other strains of Thiobacillus ferrooxidans were able to grow by hydrogen oxidation, a feature not recognized before. When cultivated on H2, a hydrogenase was induced and the strains were less extremely acidophilic than during growth on sulfidic ores. Cells of T. ferrooxidans grown on H2 and on ferrous iron showed 100% DNA homology. Hydrogen oxidation was not...
متن کاملMolecular genetics of Thiobacillus ferrooxidans.
Thiobacillus ferrooxidans is a gram-negative, highly acidophilic (pH 1.5 to 2.0), autotrophic bacterium that obtains its energy through the oxidation of ferrous iron or reduced inorganic sulfur compounds. It is usually dominant in the mixed bacterial populations that are used industrially for the extraction of metals such as copper and uranium from their ores. More recently, these bacterial con...
متن کاملAnaerobic Growth of Thiobacillus ferrooxidans.
The obligately autotrophic acidophile Thiobacillus ferrooxidans was grown on elemental sulfur in anaerobic batch cultures, using ferric iron as an electron acceptor. During anaerobic growth, ferric iron present in the growth media was quantitatively reduced to ferrous iron. The doubling time in anaerobic cultures was approximately 24 h. Anaerobic growth did not occur in the absence of elemental...
متن کاملProduction and Regeneration of Thiobacillus ferrooxidans Spheroplasts.
We investigated the production and regeneration of Thiobacillus ferrooxidans spheroplasts. Regeneration frequencies of up to 90% reversion were obtained. Attempts to transform T. ferrooxidans spheroplasts with a recombinant T. ferrooxidans plasmid were unsuccessful.
متن کاملGrowth and Maintenance of Thiobacillus ferrooxidans Cells.
A rapid and sensitive spectrophotometric procedure was developed for monitoring the growth of Thiobacillus ferrooxidans in liquid culture. Values determined for the optical densities at 500 nm of washed T. ferrooxidans cell suspensions were directly proportional to both total cell number and total cell protein concentration and provided an accurate measurement of culture growth rate. The utilit...
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ژورنال
عنوان ژورنال: The Journal of General and Applied Microbiology
سال: 1989
ISSN: 1349-8037,0022-1260
DOI: 10.2323/jgam.35.71